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KMID : 0380019950100020176
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Korean Journal of Biotechnology and Bioengineering
1995 Volume.10 No. 2 p.176 ~ p.181
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Optimal Production of Poly-β-hydroxybutyrate and Polysaccharide Methylan by Methylobacterium organophilum from Methanol
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김재연/Kim, Jae Yeon
김선원/오덕근/임현수/김정희/Kim, Sun Woo/Oh, Deok Kun/Le, Hyun Soo/Kim, Jung Hoe
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Abstract
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The environmental and physiological factors affecting the production of exopolysaccharide (Methylan) and poly-f hydroxybutyrate(PHB) by Methylobacterium organophilum were investigated. The,maximum PHB content was obtained at 38 *C whereas maximum polysaccharide concentration was 3.54g/Q at 30C. Optimum pH was pH 7-8 for PHB production and pH 6-7 for polysaccharide production, respectively. Under the condition of Mot+, Mg" or Mn" limitation with nitrogenlimitation, the PHB accumulation was increased, whereas the polysaccharide production was decreased as compared with that of solenitrogenlimitation. Under the condition of sole K+ limitation, cell growth was significantly inhibited and no polysaccharide was produced. Howeve;the PHB content was as high as 60% of dry cell weight. Effect of C/N ratios (methanol/ammonium) in the feeding solution was examined for the simultaneous production of polysaccharide and PHB. The higher ratio of C/N showed the lower cell growth, higher content of PHB in cells, and higher yield of polysaccharide.
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